| Title | Time | Room | Teacher |
|---|---|---|---|
| Scientific background Generation of a lentivirus | 28.06.2021 09:00 - 13:00 (Mon) | Urology lab H 61.01.08 | Nawroth, Roman |
| Infection of tumor cells | 28.06.2021 14:00 - 18:00 (Mon) | Urology lab H 61.01.08 | Nawroth, Roman |
| Viral vectors, cloning strategies DNA isolation from infected tumor cells Scientific background | 29.06.2021 09:00 - 13:00 (Tue) | Urology lab H 61.01.08 | Nawroth, Roman |
| Size measurement of virus particles Scientific background | 29.06.2021 14:00 - 18:00 (Tue) | Urology lab H 61.01.08 | Nawroth, Roman |
| Harvesting and Purification I | 30.06.2021 09:00 - 13:00 (Wed) | Urology lab H 61.01.08 | Nawroth, Roman |
| Infection and analysis of results I Scientific background | 30.06.2021 14:00 - 18:00 (Wed) | Urology lab H 61.01.08 | Nawroth, Roman |
| Harvesting and Purification II | 01.07.2021 09:00 - 13:00 (Thu) | Urology lab H 61.01.08 | Nawroth, Roman |
| Analysis of infection II Scientific background | 01.07.2021 14:00 - 18:00 (Thu) | Urology lab H 61.01.08 | Nawroth, Roman |
| Cell death analysis | 02.07.2021 09:00 - 13:00 (Fri) | Urology lab H 61.01.08 | Nawroth, Roman |
| Analysis and discussion of data Final discussion exam | 02.07.2021 14:00 - 18:00 (Fri) | Urology lab H 61.01.08 | Nawroth, Roman |
Level (Basic/Advanced): basic
Prerequisites for attending: none
Description of Contents:
- Theoretical background of gene delivery and gene therapy
- Safety regulations to consider before start
- Available vector systems (Oncolytic adenovirus vectors, Gene delivery: CRISPR-Cas9, shRNA delivery)
- Production and purification of adenovirus and lentivirus
- Methods for the detection of viral particles, cell lysis activity, infectivity
Learning Objectives:
Aim of this workshop is to introduce the multifaceted use of viral particles in research and therapy. Therefore, prerequisites to work with viruses, introduction into different types of viruses, how to handle a virus and the direct application to cells and analysis of these will be covered.
Exam: Oral exam at the end of the workshop
Attention: This course will also be offered in the week from 03.05.-07.05.!!!! Each time only three participants.