Cell processing and purification

Level (Basic or Advanced): basic and advanced
Prerequisites for attending (list all skills, techniques, theoretical knowledge needed to participate
in this course, especially if this is an advanced course):
None
Describe the contents and context of this course (e.g.: Are you teaching certain techniques in
the context of a specific disease or system? If so, how does that affect the way the course is taught?
Or are you teaching certain techniques as widely used tools focusing on their theoretical
background? How and why? Is the course about how to perform a technique, which techniques to
use for a certain question or about developing the experimental design? Etc.):
Flow cytometry related course with focus to clinical cell-therapy. Flow cytometry basics - also
advanced aspects and practical work included.
This workshop will cover a variety of topics related to cell processing and purification, including
advances in instrumentation and techniques, software for FCS analysis, intracellular cytokine
detection, advances in methods to measure T cell proliferation and advances in the usage of
reversible reagents. We have invited experts to discuss their work in an informal lecture setting,
discussion and workshop groups.
List the techniques that will be taught in this course:
- Flow cytometry and cell sorting and experimental setup
- Software for FCS analysis
- Data analysis/statistic
- practice on intracellular cytokine staining, methods to measure T cell proliferation and
reversible reagents, clinical cell processing
Type and Duration of Exam:
written Exam – multiple Choice – 90 minutes